E-mail: David_Kew[ ]alum.mit.edu
Hobby web page: http://capecodhistory.us/
•Education & accreditation:
Ph.D. in Biology, 1984. Wesleyan University, Middletown CT
S.B. in Life Sciences, 1976. Massachusetts Institute of Technology, Cambridge MA
Physiology Course, 1979. Marine Biological Laboratory, Woods Hole MA
Provisional licence to teach high school biology and math. Mass. Dept. Education
My scientific interests are in the broad area of developmental and cellular molecular biology. I began with a study of cellular interactions in sea urchin embryos, moving as a post-doc to cellular interactions in the rat testis, then to tissue interactions via paracrine peptide hormones in the testis, and to the hormonal feedback mechanisms at puberty. I developed and managed a small molecular biology lab with tissue culture facilities. More recently, I worked for 2 years as a contract scientist for Biogen, in several capacities, then at Brigham and Women’s Hospital, studying lung surfactant protein expression. My most recent scientific position was at GrenPharma LLC, with an Alzheimer disease project.
My hobby web site, CapeCodHistory.us, is an extension of my connection to Cape Cod, where many generations of family have lived. It comprises historical documents, literature, biographies, maps and genealogy that focus on Cape Cod, but extends to much of New England. It is the major on-line source of information about author Joseph C. Lincoln (1870-1944).
My teaching experience has been in both the lab and lecture hall. In the lab, I am pleased to show students, technicians and medical interns how to use equipment and follow experimental protocols. As a lecturer, I have experience with high school and community college students.
Integral Resources Inc., Worcester MA
Federal Census Enumerator, 2010-2011
U.S. Census Bureau
Adjunct Biology Faculty, 2010
Mount Wachusett Community College, Devens MA
Taught Human Physiology and Cell Biology
Substitute Teacher, 2006-2010
Worcester and Shrewsbury Public Schools
I have provisional certification in Massachusetts to teach Biology and Math to grades 7-12.
Senior Scientist, 2003 – 2005
GrenPharma LLC, Waltham MA
Visiting Scientist, 2003-2005
We used immunological and histological techniques to assay the effects of silicate test compounds on the diagnostic amyloid plaques of Alzhemier Disease in brain tissue, and cell culture and physical chemistry methods to model the interactions of the compounds with amyloid peptides and cells. The goal was to find promising treatments for the disease, and it resulted in several patent applications.
Research Fellow, 1999 - 2002, Pulmonary Dept.,
Brigham & Women's Hospital, Boston MA
Study of lung surfactant protein expression and regulation by cytokines in vitro. Using western blots, northern blots and immunoprecipitation to assay surfactant proteins and their gene regulatory proteins in cultured cells.
Associate Scientist, 1996-1998, Molecular Genetics Dept.,
Biogen Inc., Cambridge MA.
Used PCR to identify genes of interest. Assisted patent defense with proof-of-concept molecular cloning. Operator of ABI oligonucleotide synthesizers.
Assistant Professor of Pediatrics, 1993-1996, Tufts University School of Medicine
Scientist, 1992-1995, Neuroendocrine Research Laboratory
Lab Manager, 1993-1995, Neuroendocrine Research Laboratory
Children's Hospital, Baystate Medical Center, Springfield MA
Developed and managed a research laboratory for the Dept. of Pediatrics at Baystate Medical Center, which is the Western Campus of Tufts University Medical School. This provided students, technicians, interns and staff M.D.s with a facility for tissue culture, neuroendocrine and molecular biology projects. Conducted experiments on somatotropin-related gene expression during development in the rat testis. Taught investigators how to use equipment and follow procedures, wrote grant proposals, established a research paper database in Papyrus, obtained small animal and radiation use licenses.
Research Associate, 1990-1991, Depts. of Physiology and Urology
University of Massachusetts Medical Center, Worcester MA
Worked on neuroendocrine molecular biology projects, studying neuropeptide gene expression in the rat and mouse pituitary and testis.
Research Associate, 1986-1990, Neurobiology Group
Research Associate, 1984-1986, Endocrinology/Reproduction Group
Research Assistant, 1984, Endocrinology/Reproduction Group
Worcester Foundation for Experimental Biology, Shrewsbury MA
Studied hormonal control mechanisms in the rat testis, using techniques of cell and molecular biology, and biochemistry. We used primary tissue cultures of testicular cells, permanent cell lines and fresh tissue as sources of mRNA, neuropeptides and plasma membranes. We were particularly interested in the gene expression of the neuropeptide pro-enkephalin, which is expressed at high levels in the reproductive system and in many developing tissues.
Teaching Assistant and Research Assistant, 1976-1983, Biology Dept.
Wesleyan University, Middletown CT
Researched the developmental cell biology of sea urchin embryos, studying cell-cell adhesivity differences in distinct cell lineages. Developed techniques to isolate the distinct blastomere types in bulk, and to measure the kinetics of cell association under different conditions.
•Patent: Compositions for treating and/or preventing diseases characterized by the presence of metal ions, filed in2005, as Application No. 11/132,170. Co-inventors are Orrie Friedman, David Kew, Paul Matsudaira, Thomas Pochapsky, Neil Simister, Toby Sommer, and Julie Wei.
AW Mellon Fellowship, 1987-1989
National Research Service Award, 1984-1987
MC Chang Fellowship, 1984
Marine Biological Laboratory Scholarship, 1979
Wesleyan University teaching assistantship, 1976-1982
Orrie M. Friedman, Paul J. Matsudaira, Arthur H. Reis, Jr., Neil Simister, Ivan Correia, David Kew, Julie Y. Wei, Thomas Pochapsky (2007) Substituted Organosiloxanes as Potential Therapeutics for Treatment and Prevention of Neurodegenerative Diseases. Journal of Alzheimer Disease 11(3): 291-300
David Kew (2009) Annotated transcription of Marriage and Marriage Intention records of
from 1763 to 1850. http://CapeCodHistory.us/Wellfleet_records/Wellfleet-VRs.html Wellfleet, Mass.
David Kew (2007) Annotated transcription of the 1850 US census of
. http://CapeCodHistory.us/Wellfleet_records/Wellfleet-1850.xls Wellfleet, Massachusetts
Kew D, Muffly KE, Kilpatrick DL (1990) Proenkephalin products are stored in the sperm acrosome and may function in fertilization. Proc. National Academy of Science 87:9143-9147
Kilpatrick DL and Kew D (1990) Expression and regulation of proenkephalin gene in the testis. in: Ares-Serono Symposia, Hormonal Communication Events in the Testis. Isidori A and Dufau ML, editors. Raven Press.
Kew D and Kilpatrick DL (1990) Widespread organ expression of the rat proenkephalin gene during early postnatal development. Molecular Endocrinology 4:337-340
Kew D, Jin DE, Kim F, Laddis T, Kilpatrick DL (1989) Translational status of proenkephalin mRNA in the rat reproductive system. Molecular Endocrinology 3:1191-1196
Kew D and Kilpatrick DL (1989) Expression and regulation of the proenkephalin gene in rat Sertoli cells. Molecular Endocrinology 3:179-184
Kew D, Muffly KE and Hall PF (1986). Isolation and characterization of Sertoli cell plasma membranes. Biology of Reproduction 35:1235-1247
Osawa S, Kew D, Borland K, Krebs KE, Coleman DB, Goodman SR and Hall PF (1986). Occurrence of spectrin-like protein in Y-1 adrenal tumor cells. Endocrinology 118:2458-2463
Hall PF, Kew D, Mita M (1985). The influence of temperature on the functions of cultured Sertoli cells. Endocrinology 116:1926-1932
Borland K, Osawa S, Kew D, Coleman B, Goodman SR and Hall PF (1985). Identification of a spectrin-like protein in Sertoli cells. Biology of Reproduction 32:1143-1156
Watanabe M, Bertolini DR, Kew D, Turner RS jr. (1982). A developmental study of adhesions between cells of sea urchin embryos. Developmental Biology 91:278-285